lats1 shrna Search Results


aav-m-lats1-shrna  (Vector Biolabs)
N/A
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shrna clone set against human lats1  (Genecopoeia)
N/A
OmicsLink™ shRNA clone collections include lentiviral and non-viral vector-based shRNA constructs against genome-wide human, mouse and rat genes. shRNA of varying lengths (19 to 29 bases) were designed using a proprietary algorithm to make shRNA
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rat lats1  (OriGene)
90
OriGene rat lats1
<t>LATS1</t> inhibition with RNA interference suppresses the promoter activities of anterior pituitary hormones. (A) GH3 cells were transfected with a scrambled siRNA (control) or four different siRNA against rat Lats1 for 48 h. Immunoblot shows the knockdown efficacy and the impact of decreased LATS1 protein on YAP phosphorylation at Ser127 and total YAP and TAZ protein levels. (B) Effect of Lats1 knockdown on basal Prl and Gh promoter activity. Data are luciferase to β-galactosidase ratio, means ± standard deviation of three experiments (each in triplicate) presented as percentage of scrambled control. RLA, relative luciferase activity. * P < 0.05 to scrambled vehicle control. (C) Effect of Lats1 knockdown on forskolin-induced (10 µM, 6 h treatment) Gh promoter activity. Data are means ± standard deviation of three triplicates and presented as percentage of scrambled vehicle control. * P < 0.05 and *** P < 0.001 vs scrambled vehicle control, # P < 0.05 vs siLats1 vehicle control. Veh, vehicle – the carrier in which forskolin was diluted. (D) Lats1 knockdown does not affect cell proliferation (WST-1 colorimetric assay). Data are absorbance at OD450nm presented as percentage of scrambled siRNA control.
Rat Lats1, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lats1+shrna/Lats1+Rat+shRNA+Plasmid/pmc06215911-74-13-15
Average 90 stars, based on 1 article reviews
rat lats1 - by Bioz Stars, 2026-09
90/100 stars
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lats 1 sh rna  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology lats 1 sh rna
<t>LATS1</t> inhibition with RNA interference suppresses the promoter activities of anterior pituitary hormones. (A) GH3 cells were transfected with a scrambled siRNA (control) or four different siRNA against rat Lats1 for 48 h. Immunoblot shows the knockdown efficacy and the impact of decreased LATS1 protein on YAP phosphorylation at Ser127 and total YAP and TAZ protein levels. (B) Effect of Lats1 knockdown on basal Prl and Gh promoter activity. Data are luciferase to β-galactosidase ratio, means ± standard deviation of three experiments (each in triplicate) presented as percentage of scrambled control. RLA, relative luciferase activity. * P < 0.05 to scrambled vehicle control. (C) Effect of Lats1 knockdown on forskolin-induced (10 µM, 6 h treatment) Gh promoter activity. Data are means ± standard deviation of three triplicates and presented as percentage of scrambled vehicle control. * P < 0.05 and *** P < 0.001 vs scrambled vehicle control, # P < 0.05 vs siLats1 vehicle control. Veh, vehicle – the carrier in which forskolin was diluted. (D) Lats1 knockdown does not affect cell proliferation (WST-1 colorimetric assay). Data are absorbance at OD450nm presented as percentage of scrambled siRNA control.
Lats 1 Sh Rna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lats1+shrna/LATS1+shRNA+Plasmid/pmc05239541-192-0-7
Average 94 stars, based on 1 article reviews
lats 1 sh rna - by Bioz Stars, 2026-09
94/100 stars
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shrna hairpins against human lats1 and yap  (Broad Institute Inc)
90
Broad Institute Inc shrna hairpins against human lats1 and yap
<t>LATS1</t> inhibition with RNA interference suppresses the promoter activities of anterior pituitary hormones. (A) GH3 cells were transfected with a scrambled siRNA (control) or four different siRNA against rat Lats1 for 48 h. Immunoblot shows the knockdown efficacy and the impact of decreased LATS1 protein on YAP phosphorylation at Ser127 and total YAP and TAZ protein levels. (B) Effect of Lats1 knockdown on basal Prl and Gh promoter activity. Data are luciferase to β-galactosidase ratio, means ± standard deviation of three experiments (each in triplicate) presented as percentage of scrambled control. RLA, relative luciferase activity. * P < 0.05 to scrambled vehicle control. (C) Effect of Lats1 knockdown on forskolin-induced (10 µM, 6 h treatment) Gh promoter activity. Data are means ± standard deviation of three triplicates and presented as percentage of scrambled vehicle control. * P < 0.05 and *** P < 0.001 vs scrambled vehicle control, # P < 0.05 vs siLats1 vehicle control. Veh, vehicle – the carrier in which forskolin was diluted. (D) Lats1 knockdown does not affect cell proliferation (WST-1 colorimetric assay). Data are absorbance at OD450nm presented as percentage of scrambled siRNA control.
Shrna Hairpins Against Human Lats1 And Yap, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lats1+shrna/shrna+hairpins+against+human+lats1+and+yap/10__1158_slash_0008___5472__can___07___6205-37-7-14
Average 90 stars, based on 1 article reviews
shrna hairpins against human lats1 and yap - by Bioz Stars, 2026-09
90/100 stars
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lats1 rat shrna plasmid  (OriGene)
N/A
Lats1 Rat 4 unique 29mer shRNA constructs in retroviral untagged vector
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lats1 human shrna plasmid kit  (OriGene)
N/A
LATS1 Human 4 unique 29mer shRNA constructs in lentiviral GFP vector
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lats1 mouse shrna lentiviral particle  (OriGene)
N/A
Lats1 Mouse shRNA lentiviral particles 4 unique 29mer target specific shRNA 1 scramble control 0 5 ml each 10 7 TU ml
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lats1 human shrna lentiviral particle  (OriGene)
N/A
LATS1 Human shRNA lentiviral particles 4 unique 29mer target specific shRNA 1 scramble control 0 5 ml each 10 7 TU ml
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lats1 mouse shrna plasmid  (OriGene)
N/A
Lats1 Mouse 4 unique 29mer shRNA constructs in retroviral untagged vector
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aav-h-lats1-shrna  (Vector Biolabs)
N/A
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Image Search Results


LATS1 inhibition with RNA interference suppresses the promoter activities of anterior pituitary hormones. (A) GH3 cells were transfected with a scrambled siRNA (control) or four different siRNA against rat Lats1 for 48 h. Immunoblot shows the knockdown efficacy and the impact of decreased LATS1 protein on YAP phosphorylation at Ser127 and total YAP and TAZ protein levels. (B) Effect of Lats1 knockdown on basal Prl and Gh promoter activity. Data are luciferase to β-galactosidase ratio, means ± standard deviation of three experiments (each in triplicate) presented as percentage of scrambled control. RLA, relative luciferase activity. * P < 0.05 to scrambled vehicle control. (C) Effect of Lats1 knockdown on forskolin-induced (10 µM, 6 h treatment) Gh promoter activity. Data are means ± standard deviation of three triplicates and presented as percentage of scrambled vehicle control. * P < 0.05 and *** P < 0.001 vs scrambled vehicle control, # P < 0.05 vs siLats1 vehicle control. Veh, vehicle – the carrier in which forskolin was diluted. (D) Lats1 knockdown does not affect cell proliferation (WST-1 colorimetric assay). Data are absorbance at OD450nm presented as percentage of scrambled siRNA control.

Journal: Endocrine-Related Cancer

Article Title: Non-secreting pituitary tumours characterised by enhanced expression of YAP/TAZ

doi: 10.1530/ERC-18-0330

Figure Lengend Snippet: LATS1 inhibition with RNA interference suppresses the promoter activities of anterior pituitary hormones. (A) GH3 cells were transfected with a scrambled siRNA (control) or four different siRNA against rat Lats1 for 48 h. Immunoblot shows the knockdown efficacy and the impact of decreased LATS1 protein on YAP phosphorylation at Ser127 and total YAP and TAZ protein levels. (B) Effect of Lats1 knockdown on basal Prl and Gh promoter activity. Data are luciferase to β-galactosidase ratio, means ± standard deviation of three experiments (each in triplicate) presented as percentage of scrambled control. RLA, relative luciferase activity. * P < 0.05 to scrambled vehicle control. (C) Effect of Lats1 knockdown on forskolin-induced (10 µM, 6 h treatment) Gh promoter activity. Data are means ± standard deviation of three triplicates and presented as percentage of scrambled vehicle control. * P < 0.05 and *** P < 0.001 vs scrambled vehicle control, # P < 0.05 vs siLats1 vehicle control. Veh, vehicle – the carrier in which forskolin was diluted. (D) Lats1 knockdown does not affect cell proliferation (WST-1 colorimetric assay). Data are absorbance at OD450nm presented as percentage of scrambled siRNA control.

Article Snippet: Cells were transfected with SuperFect (Qiagen) following the manufacturer’s instructions. siRNA were against rat Lats1 (OriGene and Santa Cruz Biotechnology); a mix of scrambled non-specific siRNA was used as control.

Techniques: Inhibition, Transfection, Control, Western Blot, Knockdown, Phospho-proteomics, Activity Assay, Luciferase, Standard Deviation, Colorimetric Assay